Fig. 2.

Knockdown of circ_0101692 suppresses ccRCC cell proliferation and migration, and induced cell apoptosis in vitro, and reduced tumor xenograft growth in vivo. (A) circ_0101692 expression in KMRC-1 and KMRC-3 cells after transfection with si-circ_0101692 (si-circ) or si-NC was determined using qRT-PCR. **P < 0.01 vs. si-NC. (B) The viability of KMRC-1 and KMRC-3 cells transfected with si-circ/si-NC was detected by CCK-8 assay. **P < 0.01 vs. si-NC. (C) Apoptosis in KMRC-1 and KMRC-3 cells transfected with si-circ/si-NC was determined by caspase-3 activity assay. **P < 0.01 vs. si-NC. (D) The numbers of migratory in transfected KMRC-1 and KMRC-3 cells with si-circ/si-NC were assessed by Transwell assay. **P < 0.01 vs. the si-NC. (E) The representative solid image, tumor volume (5, 10, 15, 20, 25, 30 and 35 days), and tumor weight (at the end of 35 days) in mouse xenograft model after injection of transfected KMRC-1 cells that was stably with sh-circ/NC. **P < 0.01 vs. sh-NC.