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. 2023 Jan 4;28:101612. doi: 10.1016/j.tranon.2022.101612

Fig. 5.

Fig 5

FN1 was a target gene of miR-384. (A) TargetScan (https://www.targetscan.org/vert 80/) was used to estimate the binding site among miR-384 and FN1. (B) The target association among miR-384 and FN1 was verified by dual luciferase reporter assay. *P < 0.05, **P < 0.01 vs. WT +NC. (C) FN1 level in ccRCC and normal tissues was measured by qRT-PCR. P < 0.0001 vs. Normal. (D) FN1 level in ccRCC cell lines (KMRC-1 and KMRC-3) and HEK293 cells was detected by qRT-PCR. **P < 0.01 vs. HEK293. (E) Correlation between the expression of FN1 and miR-384 in ccRCC tissues was assessed by Pearson correlation analysis. (F) The protein levels of FN1 in KMRC-1 and KMRC-3 cells after transfection of si-FN1, si-NC, inhibitor, inhibitor-NC, or si-FN1 + inhibitor were measured by western blotting. ##P < 0.01 vs. inhibitor-NC; **P < 0.01 vs. the si-NC group; &&P < 0.01 vs. si-FN1 + inhibitor.