Fig. 2.

Effects of PM2.5 on mitophagy in vitro. Beas-2b cells were treated with different concentration of PM2.5 (50, 100, 200, 400 μg/mL). (A) Western blot analysis showed the expression levels of p-mTOR, LC3-B, ATG3 and SQSTM1/p62. (B) Autophagic flux was detected by tandem fluorescent mCherry-GFP-LC3B. (C) Western blot analysis showed the expression levels of PINK1, Parkin, TOM20 and TIM23. (D and E) Immunofluorescence assay showed the expression levels of TIM23 and TOM20. (F) Transmission electron microscopy images showed the mitophagy and mitochondrial damage. The values were showed as the mean ± SD. (**p < 0.01). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)