Figure 1.

The participation of K15⁺ and Lgr5⁺ HFSCs in SG renewal in the depilation-induced hair cycle

(A) Experimental timeline of tamoxifen/RU486 injection in 3-weeks old (3W) Lgr5:mTmG and K15:mTmG mice. P, postpartum; D, day.

(B) Representative images showing the fate of K15-lineage cells (green) during the hair cycle in 3W K15:mTmG mice.

(C) Quantitation of mGFP⁺ cells within SGs in K15:mTmG mice in the first hair cycle. Data are represented as mean ± SEM, ns, not significant, ∗p < 0.05, ∗∗∗p < 0.005, t-test, n = 3.

(D and E) Tracing of progenies of Lgr5⁺ HFSCs in Lgr5:mTmG mice after tamoxifen induction from P23 to P58 (D), and in the second hair cycle (E).

(F) Experimental scheme for RU486/tamoxifen injection and analysis of depilation-induced HF activation in K15:mTmG or Lgr5:mTmG mice.

(G) Tracing of K15⁺ HFSCs in K15:mTmG mice from P60 to P80 after RU486 induction.

(H) Quantitation of mGFP⁺ cells within SGs in 8W K15:mTmG mice at the first cycle. Data are represented as mean ± SEM, ns, not significant, ∗p < 0.05, t-test, n = 3.

(I) A schematic diagram showing the progeny of K15⁺ SCs in the depilation-induced hair cycle.

(J and K) Tracing the fate of Lgr5⁺ HFSCs in Lgr5:mTmG mice in the first (0–35 days) (J) and the second (40–75 days) (K) hair cycle following tamoxifen treatment.

(L) Quantitation of mGFP⁺ cells within SGs in 8W Lgr5:mTmG mice at the second cycle. Data are represented as mean ± SEM, ∗p < 0.05, ∗∗∗p < 0.005, t-test, n = 3.

(M) A schematic portraying the fate of Lgr5+ HFSCs in two consecutive hair cycles induced by depilation. Bu, Bulge; DP, dermal papilla; SG, sebaceous gland. Scale bars: 50 μm.