Figure 3.

Lineage tracing of multiple HFSCs for their contribution to sebocytes

(A) Lgr5 expression in telogen HF from Lgr5-GFP mouse line (left panel). The tracing of Lgr5-labeled cells in the early and mid-anagen phase (right panel) (n = 5).

(B) Immunofluorescence staining showing K15 expression in the telogen HF (left panel). In K15:mTmG mice, the progeny of K15⁺ HFSCs at early and mid-anagen were shown (right panel).

(C) Tracing of Gli1-lineage cells in 8-week-old Gli1:mTmG mice (n = 5).

(D) Tracing of Shh⁺ matrix progenitors in 8-week-old Shh:tdTomato mice after tamoxifen induction (n = 5).

(E) Counts of progenies of Lgr5⁺, K15⁺, and Gli1⁺ SCs in the SG, which were detected by the expression of mG, Data are represented as mean ± SEM, ns, not significant, ∗∗∗p < 0.005, t-test, n = 5.

(F) A schematic diagram showing the tracing of Gli1⁺ SCs during the first depilation-induced hair cycle.

(G) A schematic diagram showing the tracing of the lower bulge and upper bulge progenitors during the first depilation-induced hair cycle. Bu, bulge; LB, lower bulge; UB, upper bulge; SG: sebaceous gland; ORS, outer root sheath. Scale bars: 50 μm.