Figure 6.

Overexpression of β-catenin increases the contribution of bulge SCs to SG cells

(A–D) Tracing of Lgr5⁺ HFSCs with β-catenin activation. Representative microscopic images in low- (A) and high (B)-magnification of skin tissue sections of 8-weeks-old Lgr5:mTmG:β-catenin^act mice after hair depilation showing mGFP-labeled cells in the SG (A-B, n = 5); the contribution of Lgr5⁺ SCs to sebocytes in Lgr5:mTmG mice with normal β-catenin gene (C) or in Lgr5:mTmG:β-catenin^act mice (D) was illustrated.

(E–H) Tracing of K15⁺ HFSCs with activated β-catenin. Images showing the progenies of K15⁺ HFSCs in the SG in K15:mTmG:β-catenin^act mice at 0 days (E) and 15 days (F) after depilation (E–F, n = 5).Schematic summary of the fate of K15-lineage cells (red) in K15:mTmG (G) and K15:mTmG: β-catenin^act mice (H).

(I–K) Tracing the fate of Gli1-lineage in Gli1:mTmG:β-catenin^act mice at 15 days post-depilation. Note that most SG cells were mGFP-labeled (I-K, n = 5).

(L and M) Schematic illustrations of the fate of Gli1⁺ SCs during normal hair cycle in wild-type mice (L), and in Gli1:mTmG: β-catenin^act mice with altered expression of β-catenin (M). Telo, telogen; Ana, anagen; SG, sebaceous gland. Scale bars: 50 μm.