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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Am J Sports Med. 2022 Dec 7;51(1):81–96. doi: 10.1177/03635465221135769

Figure 5. Quadriceps protein synthesis is depressed following ACL transection in mouse quadriceps.

Figure 5.

(A) Representative image of whole murine quadriceps muscle homogenate showing puromycin-labeled peptides. (B) Quantification of puromycin band intensity in mouse quadriceps. (C) Normalized presentation of puromycin protein synthesis in the quadriceps of the ACL transected limb. (D) Representative images of type 2a fibers (blue), type 2b fibers (red) and intensity of puromycin-labeled peptides (green). (E) Normalized puromycin intensity of type 2a fibers in the quadriceps. (F) Normalized puromycin intensity of type 2b fibers in the quadriceps. Values are presented as mean ± SD with individual points overlaid. * P < .05 vs control limb. n=5-6 mice per time point. “+” denotes ACL transected (ACLT) limb, “-“ denotes healthy contralateral limb.