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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Am J Sports Med. 2022 Dec 7;51(1):81–96. doi: 10.1177/03635465221135769

Figure 8. ACL transection does not lead to robust alterations in murine myonuclear E3 ubiquitin ligase expression.

Figure 8.

A) Representative fluorescent image showing GFP-labeled myonuclei (green), that co-express PCM1 (red) in addition to being DAPI (blue) positive and residing within the dystrophin border (gray). Scale bar=50μm. B) Flow cytometry plot illustrating GFP+ nuclei co-labeled with DAPI (green square), which was used to identify and purify myonuclei via FACS. C) MuRF1 and (D) Atrogin-1 transcript read count following DESeq2 differential gene expression analysis of isolated quadriceps myonuclei. n=3 pooled biological replicates for panels C-D. “+” denotes ACL transected (ACLT) limb, “-“ denotes healthy contralateral limb.