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. 2021 Jun 29;4:100. doi: 10.1038/s42004-021-00530-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a The production process using the microfluidic double emulsion device with 50 mbar at the IA (inner aqueous solution), 57 mbar at the OA (outer aqueous solution) and 44 mbar at the LO (lipid oil). b Confocal fluorescence image of GUVs with lipid composition POPC (99.5 mol%) and DiD (0.5 mol%) obtained with only water as the IA and OA. c Size distributions of the liposomes for different flow rate configurations, showing high size monodispersity over a wide range. d Confocal fluorescence images of negatively charged GUVs with lipid composition POPC (79.5 mol%), DOPS (20 mol%), DiIC₁₈ (0.5 mol%) containing calcein (10 µM) together with the encapsulation efficiency (100%) and uniformity (RSD 6.2%) as well as their size homogeneity (67 ± 2 µm, RSD 2%).