Fig. 1.

Experimental design and dam response to the injection of the synthetic virus. A An intraperitoneal (i.p.) injection of saline or Poly I:C was performed in pregnant mice on gestational day (GD) 12.5. B Table describing all the experimental groups. C Interleukin 6 blood serum concentration (pg/ml) three hours post-injection. T ****p < 0.0001, Sal-WT vs Pol-WT *p = 0.0114 and Sal-Het vs Pol-Het **p = 0.0085. Data were analyzed by two-way ANOVA followed by a Bonferroni correction for multiple comparisons. Sal-WT n = 4, Pol-WT n = 4, Sal-Het n = 5, Pol-Het n = 5. G = Genotype of the mother; T = Treatment of the mother; and G:T = interaction between the two factors. D Weight lost (%) 24 h post-injection. Sal-Het vs Pol-Het abortion ***p = 0.0005. Data were analyzed by Kruskal–Wallis followed by Dunn’s correction for multiple comparisons. Sal-Het n = 7, Pol-Het birth n = 13, Pol-Het abortion n = 21. E Injection outcome (%). Sal-Het vs Pol-Het ****p < 0.0005. Data were analyzed by Pearson chi-square test (expected frequencies ≥ 5), two-sided. Sal-Het birth n = 16, Sal-Het abortion n = 2, Pol-Het birth n = 24, Pol-Het abortion n = 39, Pol-Het death n = 4. C–E Data were tested for normality with the Shapiro–Wilk test. Significance level was set to 0.05 (# < 0.10, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001). Mean ± SEM. ns not significant