TABLE 1.
IgG2a/IgG1a antibody responses to antigen in serum
| Clones | Immunization route | Antigen | Mean IgG2a/IgG1 ratio ×/÷ SD at wk:
|
|
|---|---|---|---|---|
| 7 | 21 | |||
| AgI/II + CT | i.n. | SBR | 0.59 ×/÷ 2.75b | 0.91 ×/÷ 1.95b |
| pSBR-CTA2/B | i.n. | SBR | 39.8 ×/÷ 8.47c | 7.07 ×/÷ 4.47c |
| pSBR | i.n. | SBR | 126 ×/÷ 2.09 | 30.2 ×/÷ 4.27 |
| pSBR-CTA2/B | i.g. | SBR | 10.7 ×/÷ 4.68 | 8.71 ×/÷ 4.79c |
| pSBR | i.g. | SBR | 13.8 ×/÷ 7.24 | 23.4 ×/÷ 2.51 |
| pSBR-CTA2/B | i.n. | Salmonella | 20.0 ×/÷ 1.66 | 15.5 ×/÷ 3.98 |
| pSBR | i.n. | Salmonella | 18.6 ×/÷ 2.14 | 20.9 ×/÷ 2.00 |
| pSBR-CTA2/B | i.g. | Salmonella | 18.6 ×/÷ 1.51 | 15.1 ×/÷ 1.82 |
| pSBR | i.g. | Salmonella | 20.4 ×/÷ 1.95 | 11.5 ×/÷ 1.51 |
a
Ratio between IgG2a and IgG1 obtained from subclass-specific ELISA. Values are presented as the geometric means ×/÷ the SD of the IgG2a/IgG1 ratios for individual mice.
b
Values significantly different (P < 0.001) from those of SBR-specific and Salmonella-specific IgG2a/IgG1 ratios in mice immunized with Salmonella clones via either the i.n. or the i.g. route.
c
Values significantly different (P < 0.001) from those of SBR-specific IgG2a/IgG1 ratios in mice immunized with the Salmonella clone expressing SBR alone via the same route.