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. 2023 Jan 5;14:1. doi: 10.1186/s13227-022-00205-5

Table 1.

Examples of genotype–phenotype mapping studies in cichlid fishes

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We included forward genetic studies that show reasonable support for a causal genotype–phenotype relationship (e.g., use independent experimental approaches, functional validation, or those phenotypes such as opsins with a very direct genotype–phenotype relationship). Targeted genotyping includes Sanger sequencing or microsatellite studies. Gene expression includes qPCR and in situ hybridization. Functional tests include CRISPR–Cas9, transgenesis, and pharmacological manipulation. QTL qualitative/quantitative trait loci mapping, GWA genome-wide association mapping