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. 2020 Nov 13;3:170. doi: 10.1038/s42004-020-00416-8

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© The Author(s) 2020

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Fig. 7 — a Methylation of 5a to give 7a; b methylation of 5b to give 7b. In all cases, the methylation system generating SAM is used to avoid the addition of stoichiometric quantities of SAM and is used with the O-methyltransferases RnCOMT and MxSafC. The methyl donor, SAM is generated in situ by the enzyme EcMAT and the reaction byproduct, SAH is broken down by another enzyme, EcMTAN.