FIG. 3.
Trypanosome DNA stimulates B-cell proliferation in a CpG-dependent manner. Positively selected B cells were cultured at 2 × 105 cells per well with 0.5 to 12.5 μg of unmethylated or methylated E. coli (A), T. cruzi (B) or T. brucei (C) DNA per ml and 10 μg of polymyxin B sulfate per ml. Results are presented as the SI of duplicate cultures of B cells cultured with DNA compared with B cells cultured with medium. These data are representative of at least two experiments performed with different cattle.