FIG. 1.

Effect of phospholipid preparation and solvents on the interaction of M. tuberculosis with Mφ. (A) Effect of soya PI and mycobacterial (myco)PI at 40 μg/ml on binding of M. tuberculosis to Mφ compared to binding medium alone. Plate Control refers to material from blank plate scrapings that had been processed identically to mycobacterial PI. The percentage of Mφ binding ≥1 and >5 M. tuberculosis is shown. The mean ± SEM is shown for four experiments, each with triplicate coverslips. ∗, P < 0.05 compared with control containing binding medium only. (B) The binding of M. tuberculosis to Mφ in the presence of 1% chloroform (CHCI₃ added) or 1% methanol (MeOH added) compared to that in the presence of binding medium sonicated in polypropylene tubes in which 100 μl of either chloroform (CHCl₃ evaporated) or methanol (MeOH evaporated) had been added and then evaporated under N₂. Control wells contained binding medium only. The percentage of the Mφ population binding ≥1 and >5 bacteria is shown. The mean ± SEM is shown for one experiment done in triplicate. ∗, P < 0.05 compared with control.