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. 2021 Jul 16;4:108. doi: 10.1038/s42004-021-00545-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Operon formed by the tdh and kbl genes in Cupriavidus necator. Genes coding tdh and kbl are colored by orange and red, respectively. LC-HRMS analysis of acetyl-CoA (b), Gly (c), and EDMP (d). The following compounds and enzymes are contained in the reaction system: L-Thr, NAD⁺, and CoA (2500 μM) (black line); L-Thr, NAD⁺, CoA (2500 μM), CnTDH, and CnKBL (red line); L-Thr, NAD⁺, CoA (100 μM), CnTDH, and CnKBL (blue line); L-Thr, NAD⁺, CnTDH, and CnKBL (green line); and standard (magenta line). Enz. means that CnTDH and CnKBL are contained in the reaction condition. b Extracted ion count chromatogram for each compound is as follows: m/z of acetyl-CoA, Gly, and EDMP were 810.1330 ± 0.0030 [M + H]⁺ (b), 76.0393 ± 0.0020 [M + H]⁺ (c), and 137.1073 ± 0.0020 [M + H]⁺, respectively. e Schematic reaction pathway to produce EDMP by CnTDH and CnKBL. Utilizing L-Thr as a starting material, CnTDH and CnKBL can generate EDMP.