FIG. 4.
IL-10 reduces the enhanced migration of T lymphocytes across B. burgdorferi-stimulated endothelium. HUVEC-amnion cultures were incubated with medium, a sham preparation of spirochetes, IL-1 (5 U/ml), or B. burgdorferi (Bb) at 10 bacteria/endothelial cell in the absence or presence of IL-10 (20 ng/ml) for 8 h. T lymphocytes (106 per culture) were then added for an additional 4 h. The total height of each bar represents the percentage of added T lymphocytes that became associated with each culture. The lower portion depicts the percentage of cells that migrated beneath the endothelium; the upper portion indicates the percentage that were adherent to the apical surface of the endothelium. Bars represent the means ± standard deviations (error bars) of three to four replicate samples. Significant reduction in migration by IL-10 is indicated by an asterisk (P < 0.01).