TABLE 2.
The population of T lymphocytes that migrates across endothelium is enriched for CD69+ cells
| Expt. No.a | Markers | % of T lymphocytes expressing indicated markers
|
|||
|---|---|---|---|---|---|
| Initial population | Migrated across unstimulated HUVECb | Migrated across IL-1-treated HUVECbcd | Migrated across B. burgdorferi-treated HUVECbd | ||
| 1 | CD4 + CD69 | 0.5 | 1.2 | 6.8 | 5.0 |
| CD8 + CD69 | 0.3 | 5.0 | 5.2 | 5.0 | |
| 2 | CD4 + CD69 | 0.3 | 2.4 | 6.6 | 5.8 |
| CD8 + CD69 | 0.4 | 6.4 | 6.3 | 6.5 | |
| 3 | CD4 + CD69 | 0.4 | 1.0 | 2.5 | 1.0 |
| CD8 + CD69 | 0.7 | 7.5 | 6.7 | 6.0 | |
a
Each experiment was performed with T lymphocytes isolated from a different donor.
b
The populations that migrated across endothelium are significantly enriched for cells coexpressing CD8 and CD69 compared to the initial population (P < 0.05).
c
The population that traversed IL-1-treated endothelium shows significant enrichment for cells coexpressing CD4 and CD69 compared to the initial population (P < 0.05).
d
In these experiments, an average of 1.9 ± 0.1-fold more T lymphocytes migrated across activated endothelium than unstimulated HUVEC.