TABLE 3.
Unstimulated and B. burgdorferi-activated endothelium preferentially recruit CD8+ T lymphocytes
| Expt. no.b | CD4+/CD8+ T-lymphocyte ratioa
|
|||
|---|---|---|---|---|
| Initial population | Migrated across unstimulated HUVECc | Migrated across IL-1-treated HUVECd | Migrated across B.burgdorferi-treated HUVECcd | |
| 1 | 4.8 | 2.1 | 4.1 | 2.7 |
| 2 | 5.0 | 2.1 | 3.1 | 2.3 |
| 3 | 1.7 | 0.8 | 1.2 | 1.0 |
| 4 | 1.1 | 0.8 | 1.1 | 0.9 |
| 5 | 1.8 | 0.8 | 1.5 | 1.3 |
| 6 | 1.5 | 0.9 | 1.2 | 0.8 |
Data are expressed as the percentage of CD4+ T lymphocytes divided by the percentage of CD8+ T lymphocytes. In these analyses, the sum of the percentages of CD4+ and CD8+ T cells averaged 99% ± 3%.
Experiments 1 and 2 used T cells isolated from the same donor on different days; the remaining experiments all used cells from different donors.
Populations that migrated across unstimulated or B. burgdorferi-treated endothelium are significantly enriched for CD8+ T lymphocytes compared to the initial population (P < 0.05).
In these experiments, an average of 2.4 ± 0.6-fold more T lymphocytes migrated across stimulated endothelium than unstimulated endothelium.