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. 2021 Nov 18;4:159. doi: 10.1038/s42004-021-00598-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a HCT116 cell captured by MFN conjugated with Cy5. Cell visualized by nuclear staining with Hoechst reagent (blue) and Cy5 (green). b Schematic of the MFN conjugated with Tf/Ab. c Immunocytochemistry method based on Fluorescein Isothiocyanate-labeled anti-cytokeratin (green), and Hoechst (blue) nuclear staining was applied to identify and enumerate CTCs captured by MFNs. PE-labeled anti-CD45 (red) and Hoechst (blue) nuclear staining was used to identify leukocytes.