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. 2022 Jun 1;5:68. doi: 10.1038/s42004-022-00685-5

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Gel image (PAGE 20%) shows the results of the TdT-mediated extension reactions with 3′-phos-dTTP 5 and 5′-FAM-labeled primer P2 (20 pmoles). Reaction mixtures contained TdT (10 U), triphosphate at 100 or 50 µM concentration, magnesium and/or manganese cofactors at 1 mM concentration, and were incubated at 37 °C for given reaction times. P represents unreacted, 5′-FAM-labeled primer.