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. 2022 Jun 1;5:68. doi: 10.1038/s42004-022-00685-5

Fig. 7. Evaluation of modified nucleotide 12 under TdT-mediated reactions.

Fig. 7

Gel image (PAGE 20%) shows the results of the TdT-mediated extension reactions with 3′-β-cyanophosphate-LNA-TTP 12 and primer P2 (20 pmoles). Reaction mixtures contained TdT (10 U), triphosphate at various, given concentrations, Co2+ (0.25 mM) or Mn2+ (1 mM) cofactors, and were incubated at 37 °C for given reaction times. P represents unreacted, 5′-FAM-labeled primer.