Table 3.
LCMS analysis of reaction products obtained with the TdT, 5′-FAM-labeled primer P2, Co2+, and 3 h of reaction at 37 °C.
| Product | m/z found (Da) | Difference (Da)a | Interpretation |
|---|---|---|---|
| Intermediate 1b | 6571.1710 | 329.0530 | Primer + 1LNA- 1H2O + 15 Da |
| n + 1 | 6556.1480 | 314.0300 | Primer + 1LNA- 1H2O |
| Intermediate 2 | 6885.2000 | 643.0820 | Primer + 2LNA- 2H2O + 15 Da |
| n + 2 | 6870.1770 | 628.0590 | Primer + 2LNA- 2H2O |
| Intermediate 3 | 7199.2290 | 957.1110 | Primer + 3LNA- 3H2O + 15 Da |
| n + 3 | 7184.2070 | 942.0890 | Primer + 3LNA- 3H2O |
| Intermediate 4 | 7513.2590 | 1271.1410 | Primer + 4LNA- 4H2O + 15 Da |
| n + 4 | 7498.2390 | 1256.1210 | Primer + 4LNA- 4H2O |
| Intermediate 5 | 7827.2870 | 1585.1690 | Primer + 5LNA- 5H2O + 15 Da |
| n + 5 | 7812.2670 | 1570.1490 | +5LNA- 5H2O |
aΔm/z with the unmodified primer P2 (6242.1180).
bbands located between those of the ni and ni+1 products.