Fig. 5. Effects of the flavone derivatives on the reactive flux towards α-synuclein oligomers.

a–g Normalised changes in ThT fluorescence when 20 µM monomeric α-synuclein with 50 nM preformed seed fibrils at pH 4.8 and 37 °C was incubated in the absence (DMSO control (black)) and presence of 10 µM of flavone derivatives, denoted by the dark coloured curve (flavone (a), 5,6,7-trimethoxyflavone (b), apigenin (c), morin (d), baicalein (e), scutellarein (f), 7-hydroxyflavone (g)). The normalised reactive flux towards oligomers, ϕ (see Eq. 8), which has a characteristic bell shape, is plotted against time and overlaid for each flavone derivative at 0.5 molar equivalents of compound, relative to α-synuclein, denoted by the light grey curve (DMSO control) and the lightly coloured curves.