TABLE 2.

Type of nucleotide substitution for exoS genes of P. aeruginosa environmental and clinical isolates compared to exoS gene of strain 388^a

Amino acid location	Codon from 388	Amino acid	Mutated codon	Resulting amino acid	Type of mutation
					Type of nucleotide substitution	Syn or Nonsynb	Codon position
46	GAC	Asp	GAG	Glu	Transversion	Nonsyn	3rd
52	GGG	Gly	GGT		Transversion	Syn	3rd
52	GGG	Gly	GGA		Transition	Syn	3rd
58	GGC	Gly	GGT		Transition	Syn	3rd
60	GCG	Ala	GCA		Transition	Syn	3rd
62	GTG	Val	ATG	Met	Transition	Nonsyn	1st
64	CCG	Pro	CCC		Transversion	Syn	3rd
74	AAA	Lys	AAG		Transition	Syn	3rd
83	GGC	Gly	GGG		Transversion	Syn	3rd
121	AGC	Ser	AAC	Asn	Transition	Nonsyn	2nd
130	CTG	Leu	TTG		Transition	Syn	1st
157	GCC	Ala	TCC	Ser	Transversion	Nonsyn	1st
159	AGC	Ser	AAC	Asn	Transition	Nonsyn	2nd
160	CAG	Gln	CAA		Transition	Syn	3rd
162	GAG	Glu	AAG	Lys	Transition	Nonsyn	1st
170	CGC	Arg	TGC	Cys	Transition	Nonsyn	1st
191	GCG	Ala	TCG	Ser	Transversion	Nonsyn	1st
212	CAG	Gln	CAA		Transition	Syn	3rd
221	CGC	Arg	CGT		Transition	Syn	3rd
266	GTG	Val	ATG	Met	Transition	Nonsyn	1st
288	CAG	Gln	CAA		Transition	Syn	3rd
289	GAG	Glu	GAA		Transition	Syn	3rd
303	GCG	Ala	GCC		Transversion	Syn	3rd
310	CAG	Gln	CAA		Transition	Syn	3rd
329	GCA	Ala	GCC		Transversion	Syn	3rd
329	GCA	Ala	GCG		Transition	Syn	3rd
387	GAA	Glu	GAG		Transition	Syn	3rd
406	GTT	Val	GTC		Transition	Syn	3rd
415	CAG	Gln	CTG	Leu	Transversion	Nonsyn	2nd
434	CGT	Arg	CCT	Pro	Transversion	Nonsyn	2nd
436	GGC	Gly	GGT		Transition	Syn	3rd
448	CGC	Arg	CGT		Transition	Syn	3rd
450	CTT	Leu	CTC		Transition	Syn	3rd

^aData from the previously published sequences of strains 388 (35) and PAO1 (15) are incorporated into this table. 

^bNonsyn and Syn, nonsynonymous and synonymous, respectively.