FIG. 1.

(A) Alignment of amino acid sequences of two conserved regions of fungal β-glucosidase reported in GenBank for Aspergillus aculeatus (Aa), H. capsulatum (Hc), Saccharomycopsis fibuligera (Sf), and Pichia anomala (Pa). (B) PCR products (a, b, and c) amplified from C. immitis genomic DNA using degenerate primers designed on the basis of conserved amino acid sequences in panel A. (C) Translated sequences of PCR products shown in panel B. The translated primer sequences are indicated (#). An asterisk indicates amino acid identity; a dot indicates a conserved substitution (see Table 2).