FIG. 5.

(A) SDS-PAGE and immunoblot analysis of E. coli-expressed rBGL2. Shown are standards (Std.), separations of lysates of transformed bacteria grown in the presence (+) or absence (−) of IPTG, nickel-affinity-isolated rBGL2 (Ni-Bd), purified rBGL2 obtained by gel electroelution (EE), and an immunoblot (Ib.) of the lysate of transformed E. coli using murine anti-rBGL2 antibody. (B) SDS-PAGE separation of detergent-extracted mycelial homogenate (My. Hm.) and corresponding immunoblot (Ib.) of native glycoprotein using anti-rBGL2 antibody.