SDS-PAGE separation of Coomassie blue-stained detergent
extracts of total parasitic cell and mycelial homogenates and results
of both immunoblot analysis and substrate gel electrophoresis (SUBST.
GEL) of these same total protein preparations. The developmental stages
of the parasitic cycle are represented by incubation time (h) after
inoculation of cultures (first generation, 16 to 132 h; second
generation, 48 h). Lane M, homogenate of 5-day mycelial culture.
Three bands are identified in the substrate gel with estimated
molecular sizes of 120, 50, and 45 kDa.