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. 2001 Apr;69(4):2211–2222. doi: 10.1128/IAI.69.4.2211-2222.2001

FIG. 8.

FIG. 8

SDS-PAGE separation of Coomassie blue-stained detergent extracts of total parasitic cell and mycelial homogenates and results of both immunoblot analysis and substrate gel electrophoresis (SUBST. GEL) of these same total protein preparations. The developmental stages of the parasitic cycle are represented by incubation time (h) after inoculation of cultures (first generation, 16 to 132 h; second generation, 48 h). Lane M, homogenate of 5-day mycelial culture. Three bands are identified in the substrate gel with estimated molecular sizes of 120, 50, and 45 kDa.