Figure 2.

Soft tissue sarcomas intratumoral microbiome at diagnosis may link with prognosis. (A) Representative gross examination of extremity synovial sarcoma specimen; asterisk demonstrates tumor necrosis and arrow identifies an encased major artery. (B) Multidimensional scaling (MDS) plot based on mathematical distance of taxonomic divergence for pre-RT intratumoral microbiome by alpha diversity. MDS plot demonstrates notable biodiversity for all pre-RT tumor samples with the exception of one patient with low intratumoral biodiversity (blue). (C–E) Relative abundances for pre-RT intratumoral microbiome by individual patients at the (C) kingdom, (D) phylum, and (E) family levels. Relative abundance describes the per cent composition of a specific organism relative to the total organism number. (F) MDS plot for pre-RT intratumoral microbiome by patient deaths. (G) MDS plot for pre-RT intratumoral microbiome by progression to metastases. (H) Kaplan-Meier estimates for overall survival (OS) and metastasis-free survival (MFS). (I–J) MDS plots for pre-RT intratumoral microbiome by (I) histology, (J) tumor size, and (K) tumor location. RT, radiotherapy.