Figure 2.

Tim-3 or VISTA traffic galectin-9 during the process of secretion. MCF-7 (A), WT3ab (B) or LN-18 (C) cells were cocultured with Jurkat T cells at a ratio of 1:1 for 16 hours. Cells were then separated and washed with fresh culture medium. Solid cancer cells were then cultured for 2 hours and galectin-9 was immunoprecipitated as described in the Materials and Methods. Eluted proteins were analyzed by Western blot in order to detect galectin-9, Tim-3 and VISTA. Images are from one experiment representative of four which gave similar results.