Table 3. Crystallographic data and refinement statistics.
| RBP-CPN | RBP-CPC | |
|---|---|---|
| PDB code | 7qsq | 7qsp |
| Wavelength (Å) | 0.9184 | 0.9184 |
| Resolution range (Å) | 48.96–1.79 (1.86–1.79) | 39.76–1.36 (1.40–1.36) |
| Space group | P21 | P212121 |
| a, b, c (Å) | 55.37, 62.77, 76.26 | 41.69, 41.97, 132.20 |
| α, β, γ (°) | 90, 102.1, 90 | 90, 90, 90 |
| Total reflections | 176181 (15604) | 533879 (48154) |
| Unique reflections | 47556 (4346) | 50883 (4875) |
| Multiplicity | 3.7 (3.6) | 10.5 (9.9) |
| Completeness (%) | 97.8 (85.5) | 99.0 (96.7) |
| Mean I/σ(I) | 8.58 (0.76) | 13.93 (1.00) |
| Wilson B factor (Å2) | 32.6 | 18.8 |
| No. of molecules in asymmetric unit | 4 | 2 |
| Matthews coefficient (Å3 Da−1) | 2.14 | 1.72 |
| R merge | 0.080 (1.324) | 0.081 (1.907) |
| R meas | 0.094 (1.548) | 0.085 (2.008) |
| R p.i.m. | 0.047 (0.788) | 0.026 (0.616) |
| CC1/2 | 0.997 (0.413) | 0.999 (0.322) |
| CC* | 0.999 (0.765) | 1.000 (0.698) |
| Reflections used in refinement | 47294 (4102) | 50883 (4875) |
| Reflections used for R free | 2088 (181) | 2100 (201) |
| R work | 0.191 (0.370) | 0.171 (0.353) |
| R free | 0.239 (0.396) | 0.210 (0.380) |
| CCwork | 0.963 (0.685) | 0.962 (0.605) |
| CCfree | 0.952 (0.532) | 0.938 (0.554) |
| No. of non-H atoms | ||
| Total | 4446 | 2308 |
| Macromolecules | 4063 | 2073 |
| Solvent | 315 | 199 |
| No. of protein residues | 510 | 248 |
| R.m.s.d., bond lengths (Å) | 0.003 | 0.012 |
| R.m.s.d., bond angles (°) | 0.57 | 1.23 |
| Ramachandran favored (%) | 98.4 | 99.2 |
| Ramachandran allowed (%) | 1.4 | 0.8 |
| Ramachandran outliers (%) | 0.2 | 0.0 |
| Rotamer outliers (%) | 1.4 | 1.4 |
| Clashscore | 5.16 | 4.82 |
| Average B factor (Å2) | ||
| Overall | 40.0 | 25.9 |
| Macromolecules | 39.2 | 24.6 |
| Solvent | 46.5 | 35.9 |
| No. of TLS groups | 4 | 2 |