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. 2023 Jan 1;79(Pt 1):1–9. doi: 10.1107/S2059798322011433

Table 2. Crystallization.

Method Sitting-drop vapour diffusion
Plate type 96-well plates
Temperature (K) 293
Protein concentration (mg ml−1) 3.44
Buffer composition of protein solution 0.1 M Bicine pH 9, 0.25 M NaCl, 5 mM MgCl2, 10% glycerol
Composition of reservoir solution 0.1 M Tris pH 8.5, 0.4 M MgCl2, 25% PEG 3350
Volume and ratio of drop (nl) 400 (1:1)
Volume of reservoir (µl) 50