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. 2022 Dec 22;13:1006998. doi: 10.3389/fimmu.2022.1006998

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Copyright © 2022 Harvey, Graves, Uppalapati, Matthews, Rosenberg, Parent, Fagerlie, Guinan, Lopez and Kronstad

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lineage gating schematic for MoDCs and NK cells. Diagram of lineage gating tree used to identify MoDCs and NK cells by flow cytometry. MoDCs and NK cells were separated into gates by size and granularity using forward and side scatter. MoDCs were further identified as CD7^- while NK cells were identified as CD7⁺CD56⁺. Cal/09 and Vic/11 infection of MoDCs at an MOI of 3 at 24 HPI is shown using an antibody specific to IAV nucleoprotein (FluA-NP). AViD, LIVE/DEAD Fixable Violet Dead Cell Stain; FSC, forward scatter; SSC, side scatter.