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. 2022 Dec 22;13:1006998. doi: 10.3389/fimmu.2022.1006998

Figure 5.

Figure 5

MoDC-NK cell cross-talk increases the frequency of an early marker of T cell activation. Summary plots of the frequency of CD69+ T cells after culture with mock-treated or Cal/09 or Vic/11-exposed MoDCs (MOI = 3) after 95 h co-culture with MoDCs and NK cells (n = 6) at a MoDC to NK cell to T cell ratio of (A) 1:1:1, (C) 4:1:1, and (E) 1:4:1 as assessed by flow cytometry using an antibody specific to CD69. Representative flow plots of the frequency of CD69+ T cells expression and MdFI after 47 h co-culture with Cal/09- or Vic/11-exposed MoDCs (MOI = 3) at a MoDC to NK cell to T cell ratio of (B) 1:1:1, (D) 4:1:1, and (F) 1:4:1. PMA/I treatment for 12 h served as a positive control. The same shape between conditions indicates that the data point is derived from the same donor. *p < 0.05, Wilcoxon signed-rank test.