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. 2023 Jan 2;12(1):2156815. doi: 10.1080/22221751.2022.2156815

Figure 6.

Figure 6.

Infected-monocytes and DCs interact with the human BBB. a Monocytes and MoDCs were infected with USUV or WNV at MOI 0.1 for 48 h and CFSE-labelled before being added on hBLECs for 30 min (for each condition, three independent experiments were analysed). hBLECs were rinsed, fixed, and analysed by microscopy. b and c CFSE labelled- and infected-monocytes (b) or MoDCs (c) were incubated on hBLECs. Indirect immunofluorescence images show immune cells (in green) and hBLECs nucleus (magenta). Scale bar 50 µm. Box plots represent quantitative analyses of CTL, USUV or WNV-infected monocyte numbers per field (20x) (*p < 0.05, **** p < 0.0001). d After 48 h of infection, MoDC mRNA were extracted. Gene expression was normalized to RPL13A and compared to mock-infected MoDCs (CTL) in the context of USUV (blue bar chart) and WNV (orange bar chart) infection. Results are expressed as mean ± SEM (n = 6, from three independent experiments). e After 48 h of infection, MoDC mRNA was extracted. Gene expression was normalized to GAPDH and compared to mock-infected MoDCs (CTL) in the context of USUV (blue bar chart) and WNV (orange bar chart) infection. Results are expressed as mean ± SEM (n = 6, from three independent experiments).