Table 1. CryoEM data collection, refinement, and validation statistics.
| Human LACTB (EMD-26595) (PDB 7ULW) |
|
|---|---|
| Data collection and processing | |
| Microscope | FEI Titan Krios |
| Camera | Gatan K3 Summit |
| Magnification | 29,000× |
| Voltage (kV) | 300 |
| Electron exposure (e-/Å2) | 65 |
| Defocus (μm) | −0.5 to −1.5 |
| Pixel size (Å) | 0.8211 |
| Symmetry imposed | Helical |
| Micrographs (no.) | 7,643 |
| Initial segment images (no.) | 430,592 |
| Final segment images (no.) | 248,548 |
| Map resolution (Å) | 3.09 |
| FSC threshold | 0.143 |
| Map resolution range (Å) | 3.1 to 5.8 |
| Refinement | |
| Initial model used | de novo |
| Model resolution (Å) | 2.84 |
| FSC threshold | 0.143 |
| Map sharpening B factor (Å2) | −87.6 |
| Model composition | |
| Nonhydrogen atoms | 18,766 |
| Protein residues | 2,400 |
| B factors (Å2)—min | |
| Protein | 78.60 |
| R.m.s. deviations | |
| Bond lengths (Å) | 0.002 |
| Bond angles (Å) | 0.406 |
| Validation | |
| MolProbity score | 1.07 |
| Clashscore | 2.84 |
| Poor rotamers (%) | 0.00 |
| Ramachandran plot | |
| Favored (%) | 98.74 |
| Allowed (%) | 1.26 |
| Disallowed (%) | 0.00 |