TABLE 1.
Percentages of TUNEL-positive bovine macrophages (apoptotic cells) infected with logarithmic- or stationary-phase Salmonella serovar Typhimurium at 0, 20, 60, and 180 min after infection
Time (min) | % of TUNEL-positive macrophages in the following samplesa:
|
||
---|---|---|---|
Uninfected control | Logarithmic phase | Stationary phase | |
0b | 14.47 ± 15.42 x | 47.75 ± 34.45 Ay | 38.51 ± 39.49 xy |
20 | 5.40 ± 6.20 x | 75.54 ± 28.34 ABy | 42.51 ± 28.88 z |
60 | 6.65 ± 5.36 x | 81.32 ± 26.97 By | 35.57 ± 31.92 x |
180 | 2.78 ± 1.88 x | 93.41 ± 5.53 By | 71.22 ± 13.39 y |
Values are means and standard deviations from five independent experiments. Different uppercase letters in the same column indicate significant difference (P < 0.05). Different lowercase letters in the same row indicate significant difference (P < 0.05).
Time zero corresponds to macrophages incubated for 30 min at 37°C after challenge with Salmonella serovar Typhimurium (see Materials and Methods).