| Antibodies |
| Alexa Fluor 488 goat-anti mouse IgG (H+L chain) |
ThermoFisher Scientific |
Cat# A11001 |
| Anti-human CD3 BV510 |
BioLegend |
Cat# 317332 |
| Anti-human CD56 BV510 |
BioLegend |
Cat# 318340 |
| Anti-human CD14 BV510 |
BioLegend |
Cat# 301842 |
| Anti-human CD27 BV605 |
BioLegend |
Cat# 302830 |
| Anti-human CD38 AF700 |
BioLegend |
Cat# 303524 |
| Anti-human CD19 ECD |
Beckman Coulter |
Cat# A07770 |
| IgG BV421 |
BD Biosciences |
Cat# 562581 |
| IgM PerCP-Cy5.5 |
BD Biosciences |
Cat# 561285 |
| E1D1 |
(Sathiyamoorthy et al., 2016) |
N/A |
| 769B10 |
(Bu et al., 2019) |
N/A |
| 769C2 |
This study |
N/A |
| 769C5 |
This study |
N/A |
| 769A7 |
This study |
N/A |
| 770F7 |
This study |
N/A |
| 770F8 |
This study |
N/A |
| |
|
|
| Bacterial and Virus Strains |
| AKATA EBV GFP |
(Molesworth et al., 2000) |
N/A |
| B95–8/F |
(Delecluse et al., 1998) |
N/A |
| Biological Samples |
| PBMC from Healthy blood bank donors |
National Institutes of Health Warren G. Magnuson Clinical Center |
N/A |
| Chemicals, Peptides, and Recombinant Proteins |
| Streptavidin PE |
ThermoFisher Scientific |
Cat# S21388
|
| Streptavidin APC |
ThermoFisher Scientific |
Cat# S32362
|
| gp42 peptide (residues 47–81) |
This paper |
GenScript Biotech Corp |
| Critical Commercial Assays |
| In Vitro Biotin Ligase kit |
Avidity |
Cat# BirA-500 |
| Superscript® III Reverse Transcriptase |
ThermoFisher Scientific |
Cat# 18080093 |
| Random Hexmers |
Gene Link |
Cat# CCL-86 |
| Igepal CA-630 |
Sigma-Aldrich |
Cat# I8896 |
| RNaseOUT |
ThermoFisher Scientific |
Cat# 10777019 |
| DNA Ligation Kits, Quick |
Takara |
Cat# 6021 |
| Britelite plus Reporter Gene Assay System |
PerkinElmer |
Cat# 6066766 |
| Expi293 Expression System Kit |
ThermoFischer Scientific |
Cat#A39250 |
| Deposited Data |
| Crystal structure gH/gL/gp42p-769C2–769B10 |
This Paper |
PDB code :7S07 |
| Crystal structure gH/gL/gp42p-769C2–770F7 |
This Paper |
PDB code: 7S1B |
| Crystal structure 769B10 Fab |
This paper |
PDB code: 7S08 |
| Crystal structure 770F7 Fab |
This paper |
PDB code: 7S0J |
| EM gH/gL-769B10 |
This Paper |
|
| EM gH/gL-769A7 |
This Paper |
|
| EM gH/gL-769C2 |
This Paper |
|
| EM gH/gL-769F8 |
This Paper |
|
| EM gH/gL-769C5 |
This Paper |
|
| 769A7 |
This Paper |
OP453131/ OP453132
|
| 769C2 |
This Paper |
OP453123/ OP453124
|
| 769C5 |
This Paper |
OP453129/ OP453130
|
| 769F7 |
This Paper |
OP453125/ OP453126
|
| 769F8 |
This Paper |
OP453127/ OP453128
|
| Experimental Models: Cell Lines |
|
|
| 293T |
ATCC |
Cat# ACS-4500 |
| CHO-K1 |
ATCC |
Cat# CCL-61 |
| Raji |
ATCC |
Cat# CCL-86 |
| Expi293F |
ThermoFisher Scientific |
Cat# A14527 |
| Daudi |
(Silva et al., 2004) |
N/A |
| 293T-14 |
(Omerović et al., 2005) |
N/A |
| SVKCR2 |
(Li et al., 1992) |
N/A |
| 293/2089 |
(Delecluse et al., 1998) |
N/A |
| Akata BX1 |
(Molesworth et al., 2000) |
N/A |
| Experimental Models: Organisms/Strains |
| Female HSCCB-NOG-F (NOD.Cg-Prkdcscid
Il2rgtm1Sug/JicTac) mice |
Taconic |
N/A |
| female and male C57BL/6, B6. mFcRn−/− hFcRn Tg32 mice |
Jackson Laboratory |
N/A |
| Recombinant DNA |
| pCAGGS EBV gH |
(Silva et al., 2004) |
N/A |
| pCAGGS EBV gL |
(Silva et al., 2004) |
N/A |
| pCAGGS EBV gB |
(Silva et al., 2004) |
N/A |
| pCAGGS EBV gp42 |
(Silva et al., 2004) |
N/A |
| pCMV/R-gH-AviHis |
(Bu et al., 2019) |
N/A |
| pCMV/R-gL |
(Bu et al., 2019) |
N/A |
| Software and Algorithms |
| Flowjo |
Flowjo |
http://www.flowjo.com
|
| Prism |
Graphpad |
http://www.graphpadcom
|
| Biorender |
Biorender |
http://www.biorender.com
|
| Pymol |
Schrödinger, Inc. |
https://pymol.org/2/
|
| phenix.refine |
Phenix software suite |
https://phenix-online.org/
|
| Coot |
MRC Laboratory of Molecular Biology in Cambridge |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
|
| Other |
|
|
| Captia EBV VCA (P-18) IgG Kit |
Trinity Biotech |
Cat# 2324700 |
| Ni-NTA agarose |
ThermoFisher Scientific |
Cat# R901–15 |
| Tecnai™ T20 |
FEI Company |
N/A |
| Streptavidin biosensors |
FortéBio |
Cat# 18–5019 |
| Octet HTX instrument |
FortéBio |
N/A |
