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. 2023 Jan 5;8(1):40–54. doi: 10.1038/s41564-022-01284-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Gains and losses in LCCA relative to LVCCA are indicated by blue and red backgrounds, respectively. The reconstructed presence of a peptidoglycan-based cell wall is indicated by the dashed line between inner and outer membranes. ADI, arginine deiminase pathway; CI–V, electron transport chain complexes I–V; Hyd, [FeFe]-hydrogenase; PDC, pyruvate dehydrogenase; T4P, type IV pilus. See also Extended Data Fig. 2 for a more detailed summary and Supplementary Data 9 for gene content reconstructed in LCCA and LVCCA.