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. 2023 Jan 5;8(1):40–54. doi: 10.1038/s41564-022-01284-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a,b, Proteome size inferred for chlamydial ancestors, and protein-coding gene copies present in extant chlamydial genomes. a, Inferred proteome size of early chlamydial ancestors scaled to relative evolutionary divergence, from LCCA to extant taxa. b, Proteome size comparison between inferred ancestors and extant members within each chlamydial family. Chlamydiae without in-family ancestors are grouped together in the grey boxplot (that is, Criblamydiaceae, Waddliaceae, Sororchlamydiaceae and unclassified). Points and boxplots are coloured according to the legend. Centre lines in the box-and-whisker plot represent median values, box limits represent upper and lower quartile values and whiskers represent 1.5 times the interquartile range above the upper quartile and below the lower quartile. Number of ancestors and extant members per family depicted from left to right: n = 14, 16, 14, 16, 4, 6, 6, 8, 17, 19, 12, 14 and 12. Abbreviations of key ancestors are labelled as in Fig. 3. See also Supplementary Data 7 for proteome sizes.