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. 2023 Jan 5;8(1):40–54. doi: 10.1038/s41564-022-01284-9

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Genes were reconstructed as present if they had an inferred relative frequency ≥0.3 in ALE. Enzyme complexes were inferred as present if at least half of the necessary genes were present. Enzymes are annotated with enzyme commission numbers, arrows indicate the directionality of reactions and are coloured in black or grey if inferred as present or absent, respectively. Highlights show changes in inferred gene content between LVCCA and LCCA. Purple, light purple and red highlights indicate inferred gains, presence (while not inferred in LVCCA), and losses LVCCA, respectively. See Data S9 for gene families inferred in LCCA and LVCCA and corresponding annotations.