TABLE 2.
Numbers of influenza virus-specific AFC per 106 cells from mice after intranasal immunization using the mucosal adjuvant CT
| Tissue | Isotype | Value for amt of CT (μg)/intranasal immunizationa
|
i.p.b | |||
|---|---|---|---|---|---|---|
| 0 | 0.1 | 0.5 | 2.5 | |||
| Lung | IgM | 6 (3.0)c | 84 (1.42) | 73 (2.8) | 70 (1.6) | 3 |
| IgG | 14 (1.6) | 344 (1.0) | 348 (1.1) | 1,074 (3.0) | 1 | |
| IgA | 12 (1.1) | 2,576 (1.6) | 2,871 (1.9) | 2,965 (1.8) | 0 | |
| Nasal\passages | IgM | 3 (3.5) | 15 (1.3) | 15 (1.5) | 9 (1.6) | 2 (2.2) |
| IgG | 1 (1.6) | 196 (1.8) | 251 (1.8) | 189 (1.4) | 5 | |
| IgA | 32 (1.1) | 3,319 (1.5) | 7,161 (1.4) | 11,940 (1.2) | 0 | |
| Spleen | IgM | 15 (1.0) | 27 (1.1) | 34 (1.2) | 48 (1.2) | 95 (1.7) |
| IgG | 2 (1.2) | 61 (2.8) | 93 (4.6) | 139 (3.0) | 1.3 (1.9) | |
| IgA | 2 (1.9) | 292 (1.3) | 407 (1.9) | 614 (1.2) | 0 | |
a
Mice were intranasally immunized weekly (four times) with 7.5 μg of influenza virus vaccine in combination with various doses of CT. Six days after the last immunization, the numbers of specific-antibody-producing cells per 106 cells from each of the tissues were determined.
b
Mice were intraperitoneally (i.p.) immunized weekly (four times) without CT.
c
Values are geometric means (± standard errors) of the numbers of influenza virus antigen-specific AFC per 106 viable cells of the specified isotype. Results are taken from two experiments (five mice in each group for each experiment).