TABLE 3.
Total numbers of influenza virus-specific AFC from each tissue obtained from mice after intranasal immunization using the mucosal adjuvant CT
| Tissue | Isotype | Value for amt of CT (μg)/intranasal immunizationa
|
i.p.b | |||
|---|---|---|---|---|---|---|
| 0 | 0.1 | 0.5 | 2.5 | |||
| Lung | IgM | 18 (2.5)c | 1,188 (2.3) | 1,334 (1.0) | 1,698 (2.1) | 10 |
| IgG | 41 (1.8) | 15,382 (1.1) | 20,091 (1.3) | 26,182 (1.1) | 3 | |
| IgA | 34 (1.0) | 36,392 (2.0) | 52,481 (1.4) | 72,444 (1.8) | 0 | |
| Nasal passages | IgM | 3 (3) | 7.7 (1.3) | 7 (1.9) | 5 (1.1) | 2 (1.9) |
| IgG | 1 (1.6) | 94 (1.8) | 121 (2.4) | 114 (1.0) | 3 (1.1) | |
| IgA | 27 (1.0) | 2,296 (2.2) | 3,656 (2.5) | 3,793 (1.1) | 0 | |
| Spleen | IgM | 927 (1.6) | 1,660 (1.3) | 2,455 (1.3) | 2,399 (1.5) | 3,698 (1.9) |
| IgG | 147 (1.3) | 3,690 (3.4) | 6,607 (5.0) | 7,031 (4) | 662 (2.1) | |
| IgA | 10 (12) | 17,620 (1.6) | 28,973 (2.2) | 31,046 (1.7) | 13 (17) | |
Mice were intranasally immunized weekly (four times) with 7.5 μg of influenza virus vaccine in combination with various doses of CT. Six days after the last immunization, the total numbers of specific-antibody-producing cells in the cells isolated from each of the tissues were determined.
Mice were intraperitoneally (i.p.) immunized weekly (four times) without CT.
Values are geometric means (± standard errors) of the total numbers of influenza virus antigen-specific AFC of the specified isotype obtained from a tissue. Results are taken from two experiments (five mice in each group for each experiment).