TABLE 5.
Numbers of influenza virus-specific AFC per 106 cells from each tissue obtained from mice after intranasal immunization
| Tissue | Isotype | Value for route of immunizationa
|
||||
|---|---|---|---|---|---|---|
| Control | IP:0 | 0:IN | IP:IN | IN:IN | ||
| Nasal passages | IgA | 0b | 2 (2.2) | 63 (1.5) | 157 (1.3) | 3,597 (1.2)* |
| IgG | 0 | 17 (4.7) | 0 | 13 (4.2) | 66 (9.4) | |
| IgM | 0 | 22 (5.2) | 7 (3.1) | 121 (1.4) | 7 (3.1) | |
| Lung | IgA | 0 | 10 (1.7) | 3 (1.2) | 67 (2.6)* | 323 (1.7)* |
| IgG | 0 | 6 (1.7) | 9 (1.2) | 66 (1.4)* | 27 (1)* | |
| IgM | 0 | 3 (1.5) | 5 (1.3) | 17 (2.1)* | 26 (1.1)* | |
| Spleen | IgA | 2 (1.7) | 5 (1.9) | 10 (1.4) | 16 (1.3) | 899 (1.4)* |
| IgG | 2 (1.5) | 26 (2.2) | 10 (1.5) | 69 (1.1) | 265 (1.3)* | |
| IgM | 11 (1.9) | 38 (1.5) | 53 (1.1) | 59 (1.1) | 344 (1.2)* | |
a
Immunization groups are designated as described in Table 4. All immunizations were performed with inactivated influenza virus vaccine plus 0.1 μg of CT.
b
Values are geometric means (± standard errors) of numbers of AFC per 106 cells from three experiments (two experiments for lung IgG and IgM) with five mice each. Asterisks indicate values that are significantly different from those for both primary immunization groups (0:IN and IP:0).