FIG. 3.

Effect of IL-10 and TGF-β1 on TNF production. (A and B) Monocytes were pretreated with IL-10 or TGF-β1 (5 ng/ml) and then incubated in the absence (A) or the presence (B) of C. burnetii (bacterium-to-cell ratio, 200:1) for 4 h. Total RNA was extracted and transcribed in cDNA. After amplification, PCR products were analyzed by agarose gel electrophoresis and ethidium bromide staining. The figure is representative of three experiments. (C) Monocytes were pretreated with IL-10 or TGF-β1 for 24 h and then infected with C. burnetii. Supernatants were assayed for the presence of TNF by ELISA after different times. The results are expressed as the mean ± SE of three experiments.