FIGURE 4.

CircZSWIM6 interacts with ribosomal protein S14 (RPS14) and affects RPS14 ubiquitylation: (A) sliver staining of proteins binding to CircZSWIM6; (B) RNA pull‐down is performed using control probe and CircZSWIM6 probe, followed by western blotting using RPS14 antibody; (C) CircZSWIM6 is co‐localized with RPS14 in human chondrocyte; (D) prediction of the regions of CircZSWIM6 interacts with RPS14; (E) binding sequence of RPS14 for CircZSWIM6 identified by RNA immunoprecipitation (RIP) assay; (F) illustration of RPS14 fragments; (G) binding sequence of CircZSWIM6 for RPS14 via RIP assay; (H) RPS14 protein level after CircZSWIM6 knock‐down or overexpression indicated by western blot analysis; (I) mRNA level of RPS14 after CircZSWIM6 knock‐down or overexpression; (J and K) RPS14 protein level in human chondrocyte treated with the transcription inhibitor CHX with or without CircZSWIM6 knock‐down; (L) effect of bortezomib (Borz) on RPS14 protein level after CircZSWIM6 knock‐down; (M) ubiquitylation of RPS14 in human chondrocyte with CircZSWIM6 knock‐down or overexpression; quantitative data shown as mean ± SD; exact p‐values are shown in figures. Two‐side unpaired Student's t‐test was used for statistical analysis. One‐way ANOVA with Tukey's multiple comparison was used for statistical analysis.