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. 2022 Dec 23;13:1064328. doi: 10.3389/fmicb.2022.1064328

Table 1.

Ingredient compositions of experimental diets1.

Ingredient, % Control, phase I Control, phase II
Corn 44.41 57.27
Dried whey 15.00 10.00
Soybean meal 18.00 22.00
Fish meal 10.00 7.00
Lactose 6.00
Soy protein concentrate 3.00
Soybean oil 2.00 2.00
Limestone 0.56 0.70
L-Lysine·HCl 0.21 0.23
DL-Methionine 0.08 0.05
L-Threonine 0.04 0.05
Salt 0.40 0.40
Vit-mineral, Sow 62 0.30 0.30
Total 100.00 100.00
Calculated energy and nutrient
Metabolizable energy, kcal/kg 3,463 3,429
Net energy, kcal/kg 2,601 2,575
Crude protein, % 22.27 20.80
Arg,3 % 1.23 1.15
His,3 % 0.49 0.47
Ile,3 % 0.83 0.76
Leu,3 % 1.62 1.55
Lys,3 % 1.35 1.23
Met,3 % 0.45 0.39
Thr,3% 0.79 0.73
Trp,3 % 0.23 0.21
Val,3 % 0.91 0.84
Met + Cys,3 % 0.74 0.68
Phe + Tye,3 % 1.45 1.38
Ca, % 0.80 0.70
Total P, % 0.68 0.59
Digestible P, % 0.47 0.37
Analyzed nutrient, as-is
Dry matter, % 90.70 89.90
Crude protein, % 23.13 21.30
ADF, % 7.26 9.35
NDF, % 2.54 3.60
Ca, % 0.96 0.88
P, % 0.71 0.59

1In each phase, two additional diets were formulated by adding probiotics or Carbadox to the control diet, respectively. The dose for probiotics was 500 mg/kg, which was equal to 2.56 × 109 CFU Bacillus subtilis/kg diet. The dose for carbadox was 50 mg/kg diet.

2Provided the following quantities of vitamins and micro minerals per kilogram of complete diet: Vitamin A as retinyl acetate, 11,136 IU; vitamin D3 as cholecalciferol, 2,208 IU; vitamin E as DL-alpha tocopheryl acetate, 66 IU; vitamin K as menadione dimethylprimidinol bisulfite, 1.42 mg; thiamin as thiamine mononitrate, 0.24 mg; riboflavin, 6.59 mg; pyridoxine as pyridoxine hydrochloride, 0.24 mg; vitamin B12, 0.03 mg; D-pantothenic acid as D-calcium pantothenate, 23.5 mg; niacin, 44.1 mg; folic acid, 1.59 mg; biotin, 0.44 mg; Cu, 20 mg as copper sulfate and copper chloride; Fe, 126 mg as ferrous sulfate; I, 1.26 mg as ethylenediamine dihydriodide; Mn, 60.2 mg as manganese sulfate; Se, 0.3 mg as sodium selenite and selenium yeast; and Zn, 125.1 mg as zinc sulfate.

3Amino acids were indicated as standardized ileal digestible AA.