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. 2022 Dec;10(23):1278. doi: 10.21037/atm-22-5273

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Autophagosomes were observed using electron microscopy. Rat spinal cord samples were collected from the four groups (SNI + EA, SNI + EA + DMSO, SNI + EA + AMPK inhibitor, and SNI) on day 14 post-surgery following quantification of the paw withdrawal threshold. Electroacupuncture-induced autophagosome formation was observed in the spinal microglial cells, which was inhibited by intrathecal injection of the AMPK inhibitor. Autophagosomes were represented by the red arrows. Scale bar, 2 µm. SNI, spared nerve injury; EA, electroacupuncture; AMPK, AMP-activated protein kinase; DMSO, dimethylsulfoxide.