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. 2022 Dec;10(23):1280. doi: 10.21037/atm-22-5444

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2022 Annals of Translational Medicine. All rights reserved.

Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0.

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Knockdown of TMED2/3/4/9 remarkably inhibited the migration and invasion of breast cancer in vitro. (A) The relative expressions of TMED2/3/4/9 in different breast cancer lines (B) The efficiency of siRNA transfection was validated by western blot assay. The protein levels of TMED2/3/4/9 were remarkably down-regulated in both MDA-MB-231 and HCC1937 cell lines. (C) The migration capabilities of MDA-MB-231 and HCC1937 cells after siRNA transfection were determined by wound healing assay. (D) Cell migration abilities of MDA-MB-231 and HCC1937 cells transfected siRNAs were evaluated by transwell invasion assays. The invading cells on the basal side of the chambers were dyed with 0.1% crystal violet. Each experiment was repeated at least 3 times independently with the similar results. *, P<0.05; **, P<0.01; ***, P<0.001. TMED, transmembrane p24 trafficking protein; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; siRNA, small interfering RNA; NC, negative control group; KD, knockdown group.