Table 4.

Behavioral patterns of Nile tilapia (Oreochromis niloticus) fish subjected to the heavy metals, mercury (Hg) and cadmium (Cd), and treated with biosynthesized selenium nanoparticles (BioSeNPs) during the 10 week experimental period.

Groups	Subgroups	Cd + Hg(μg ml−1)	BioSeNPs(μg ml−1)	Feeding time (sec)	Feeding frequency	Foraging time (sec)	Foraging frequency	Surfacing time (sec)	Surfacing frequency	Swimming time (sec)	Swimming frequency	Resting time (sec)	Resting frequency	Arousal time (sec)	Arousal frequency	Aggression time (sec)
1	1Z	–	–	120.0 g	6.0e	140.0a	4.0c	220.0e	9.2ab	1180.0e	25.0c	400.0b	7.0c	900.0f	23.0d	190.0a
	1A	–	3	160.0d	7.2c	130.0d	3.8 cd	220.0e	8.5b	1255.0d	25.4c	405.0b	7.5c	1125.0c	24.0c	65.0d
	1B	–	4	175.2c	8.9b	124.0e	3.1d	219.0f	7.3c	1354.0c	26.9b	410.0ab	8.4ab	1156.0b	25.1b	42.0f
	1C	–	5	192.0a	10.0a	120.0f	2.5e	221.0e	6.8d	1500.0a	28.0a	420.0a	8.8a	1200.0a	29.0a	25.0 g
2	2Z	0.5 + 0.5	–	104.0 h	6.4d	138.0b	5.1ab	250.0ab	9.1ab	1104.0f	24.0d	300.0e	6.5d	996.0e	23.6d	135.0b
	2A	0.5 + 0.5	3	145.0e	6.1e	135.0c	4.8b	231.0c	8.2bc	1198.0e	24.0d	320.0d	7.0c	1025.0d	24.0c	70.0d
	2B	0.5 + 0.5	4	167.0 cd	7.2c	130.0d	4.0c	226.0d	7.7c	1289.0d	25.1c	340.0c	7.5c	1105.0c	24.2c	56.0e
	2C	0.5 + 0.5	5	185.0b	8.2bc	128.0e	3.2d	220.0e	7.0d	1405.0b	26.0b	380.0c	8.0b	1150.0b	25.0b	30.0 g
3	3Z	1.0 + 1.0	–	78.0i	6.7d	141.0a	5.5a	255.0a	9.7a	1090.0f	24.0d	290.0e	6.0e	998.0e	23.0d	90.0c
	3A	1.0 + 1.0	3	135.0f	6.0e	137.0b	4.5b	240.0b	8.9b	1150.0e	24.7c	310.0d	6.4e	1011.0d	24.2c	87.0 cd
	3B	1.0 + 1.0	4	151.0d	6.4d	131.0c	4.1c	235.0c	8.2bc	1240.0d	25.0c	325.0d	6.9d	1089.0d	24.8c	67.0d
	3C	1.0 + 1.0	5	167.0 cd	7.0c	129.0e	3.9 cd	230.0c	7.9c	1350.0c	25.8c	350.0c	7.5c	1120.0c	26.0b	42.0f

Means in the same column with lowercase different letters are significantly different at p ≤ 0.05.